Recombinant Human CD47/IAP Protein (His Tag)(Active)

Recombinant Human CD47/IAP Protein (His Tag)(Active)

SKU #	PKSH033600
Expression Host	HEK293 Cells

Description

Synonyms	Antigenic Surface Determinant Protein OA3,CD47,IAP,Integrin-Associated Protein,Leukocyte Surface Antigen CD47,MER6,Protein MER6
Species	Human
Expression Host	HEK293 Cells
Sequence	Gln19-Pro139
Accession	Q08722
Calculated Molecular Weight	14.8 kDa
Observed Molecular Weight	30-45 kDa
Tag	C-His
Bio-activity	Loaded Anti-Human CD47 mAb-Fc on Protein A Biosensor, can bind Human CD47-His with an affinity constant of 0.22 nM as determined in BLI assay.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin	< 1.0 EU per g of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80. Reconstituted protein solution can be stored at 4-8 for 2-7 days. Aliquots of reconstituted samples are stable at < -20 for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 m filtered solution of 10mM Tris-Citrate, 150mM NaCl, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background
CD47(Integrin-Associated Protein;IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages; neutrophils; and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin; and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution; and is reduced in expression on Rh erythrocytes.